DS 29190 PDF

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We may receive commission if your application for credit is successful. See all condition definitions – opens in a new window or tab Defining a region of caveolin that contains c-Src binding activity. This amino acid stretch of caveolin residues is located within a membrane-proximal region 29910 the cytosolic N-terminal domain of caveolin.

However, the tightly regulated tyrosine kinase activity of c-Src can be activated transiently by a number of growth factors 38 Using either Triton-based methods 121314151617 or detergent-free methods 181920it has been demonstrated that caveolin co-purifies with cytoplasmic lipid-modified signaling molecules. The effects of peptide 1 Apeptide 2 Band peptides 3 and 4 C are shown. Philipp Scherer for critical discussions and help with protein quantitation; Dr.

Baculovirus constructs encoding either c-Src or v-Src from chicken were the generous gifts of Drs.

Pdyn (prodynorphin) | Gene Report | BioGPS

Thus, a 2990 mammalian cell, such as smooth muscle cells or fibroblasts, may co-express up to three or four immunologically distinct caveolin protein products. Add to Watch list Watching. Note that peptide 1 had no effect, whereas peptide 2 dose-dependently suppressed the auto-activation of Fyn. The effects of these peptides 291900 shown in B.


The SH2 domain recognizes specific phosphotyrosine based motifs 61whereas the SH3 domain binds proline-rich peptide sequences with the consensus P XX P 6263 Quantitation was performed as detailed previously Quantitation of these experiments is provided in D.

This is consistent with results demonstrating co-immunoprecipitation of caveolin with c-Src using antibodies directed against c-Src Fig. As a consequence of 2910 divergence, v-Src does not contain Tyrwhich when phosphorylated can act to functionally inactivate c-Src kinase The expression of Src tyrosine kinases was 2990 by immunoblot analysis with a mAb that recognizes the conserved extreme N-terminal of Src.

However, similar fusion containing caveolin residues did not show any interaction.

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Using an established in vitro binding assay, we find that caveolin interacts with wild-type Src c-Src but does not form a stable complex with mutationally activated Src v-Src. In addition, caveolin prefers the inactive conformation of these molecules, and caveolin binding can hold these molecules in the inactive conformation. Caveolin was recombinantly expressed as a glutathione S -transferase fusion. Learn More – opens in a new window or tab.

Kunxin Luo Whitehead Institute and Dr. Related Content Load related web page information.

The inhibitory effect of peptide 2 was slightly less potent on Fyn tyrosine kinase. To examine the functional consequences of the interaction of caveolin with Src tyrosine kinases, we generated a panel of caveolin peptides that span the region of caveolin. Previous Section Next Xs.

These scaffolding proteins, Ste5p 7374AKAP 293075762910 77787980 may simultaneously associate with distinct classes of signaling proteins to es a signaling pathway or module. Because mutations in the extreme C terminus, the kinase domain, the SH2 domain, or the SH3 domain can constitutively activate c-Src, this activation may be mediated a conformational change in one of these regions Most purchases from business sellers are protected by the Consumer Contract Regulations which give you the right to cancel the purchase within 14 days after the day you receive the item.


Accepted, Eligibility for PayPal Credit is determined at checkout. Mutationally 2919 c-Src has been implicated in the pathogenesis of carcinomas, including breast tumors See all carpartsinmotion has no other items for sale.


Add to Watch list. Both classes of tyrosine kinases can function as transforming oncogenes when mutationally activated 50 For additional information, see the 291190 Shipping Programme terms and conditions – opens in a new window or tab.

Add to basket. Briefly, full-length caveolin residuesthe C-terminal domain of caveolin residuesand regions of the N-terminal domain of caveolin residues,and were subcloned into the vector pGEX-4T Please enter a number less than or equal to 2. Construction and Purification of Glutathione S-Transferase-Caveolin Fusion Proteins The construction, expression, 2190 purification of GST-caveolin 1 fusion proteins were as we described previously 2425 ,